Octet® R8e BLI system for label-free characterization of biomolecules
Octet® R8e is a BLI system enhanced with features such as increased sensitivity, flexible assay design, and extended unattended runtime. Utilizing improved Bio-Layer Interferometry (BLI), it transforms biomolecular interaction analysis and accelerates biotherapeutic development. Its improved signal-to-noise ratio enables accurate kinetic characterization of low molecular weight analytes such as small molecules and proteins, as well as detection of analytes at low concentrations.
Octet® R8e BLI System Features
- Next-level sensitivity – Best-in-class sensitivity with a higher signal-to-noise ratio for precise detection of low molecular weight and low-concentration analytes
- Fast data acquisition – Enhanced data collection captures key data points for reliable kinetic measurements, even at high binding rates
- Evaporation control – Maintains analyte concentration stability for up to 16 hours, allowing accurate data generation during extended or overnight experiments
- Flexibility – Run up to 8 assays in parallel; easy assay transferability; compatible with both 96- and 384-well plates; parallel processing starting at just 40 μL sample volume preserves valuable samples and reduces reagent costs
- Fluidics-free design – Unmatched time and cost savings through simplified system setup and operation
- Extended unattended operation – More than 16 hours of autonomous run time
Cost Efficiency and Increased Productivity
The Octet® R8e BLI system supports both 96-well and 384-well microplates. In workflows involving expensive or scarce analytes, the 384-well format offers substantial cost savings and enables higher sample throughput per plate.
- Minimal sample volume – As low as 40 µL per well
- Increased productivity – Higher sample throughput per plate
- Greater efficiency – Fewer plate exchanges for more results in a single analytical session
Limitless Application Potential
The Octet® BLI platform supports a wide range of applications:
- Competition and inhibition assays
- Off-rate ranking
- Glycan profiling
- Production monitoring
- Bioprocess quality control (QC)
- DNA/RNA – protein binding
- Fc receptor binding
- Virus/vaccine studies
- Aptamer screening
- Ligand binding assays
- Biosimilar analysis
- ELISA replacement
- Small molecule interaction
- AAV E/F capsid ratio
- Epitope binning
- Host cell protein (HCP) analysis
Sensorgram Comparison: Octet® R8e vs Octet® R8
The Octet® R8e BLI system achieves picometer resolution, enabling precise investigation of weak molecular interactions at low ligand densities. This reduces secondary interactions, preserves analyte activity, and minimizes mass transport effects, allowing researchers to make informed decisions in discovery programs while ensuring compliance with 21 CFR Part 11 regulations.
Frequently asked questions
The Octet® R8e features advanced evaporation control for the 96-well plate format, allowing for up to 16 hours of uninterrupted operation while maintaining analyte integrity, reducing the need for manual intervention, and improving data accuracy. This means the system delivers consistent, reliable results even during long or over-night experiments.
The Octet® R8e System is a cutting-edge, 8-channel biolayer interferometry (BLI) system designed for real-time, label-free biomolecular analysis. It features a fluidic-free design that minimizes maintenance, simplifies workflows, and ensures consistent data accuracy. The Octet® R8e boasts best-in-class sensitivity, extended walk-away operation, and supports GxP compliance. With its high throughput and robust performance, the system is ideal for complex drug discovery and process development applications.
With compatibility for both 96- and 384-well plates, the Octet® R8e is designed to support high-throughput workflows. The system’s 8 channels enable parallel data acquisition, allowing labs to process multiple samples simultaneously, boosting efficiency and maximizing productivity.
Yes, the Octet® R8e is designed for seamless assay transferability across platforms. Methods developed on older Octet® BLI systems can be easily transferred without compromising data integrity or comparability, ensuring smooth transitions and consistent results across your lab’s operations.
The Octet® R8e requires as little as 40 µL of sample per well, making it highly efficient and cost-effective, especially when working with precious or limited sample quantities. This low sample volume capability helps conserve reagents and minimize costs. Furthermore, the measurement is non-destructive and the sample plate can be recovered for further usage.
