Classical Media

MEM Medium

MEM was developed by Harry Eagle, and is one of the most widely used basal media for synthetic cell culture media formulations. MEM is a modification of Basal Medium Eagle (BME), containing a higher concentration of essential nutrients than BME. MEM is frequently used to cultivate a variety of cells grown in monolayers.

DMEM Medium

DMEM is a modification of Basal Medium Eagle (BME), with a higher concentration of amino acids and vitamins than BME and additional supplemental components. DMEM is one of the most common basal media used in cell culture, and is the basis for many specialty media and serum-free
formulations.

RPMI Medium

RPMI 1640 Medium was specifically developed for the long-term culture of blood cells, including normal and abnormal human leukocytes (e.g. neoplastic white blood cells). With the addition of serum, RPMI 1640 is also used as a general culture medium for hybridomas

Ham‘s Nutrient Mixtures

Ham’s F-10 and F-12 Nutrient Mixtures were originally developed for serum-free culture of CHO cells, and are now standard reagents in a cell culture lab. Ham’s F-10 and F-12 are used for a variety of cell culture applications with and without the addition of a serum supplement.

Laboratory Waters and Buffers for Cell Culture

Sartorius offers a wide range of laboratory-grade waters and buffers essential for maintaining cell health and viability and ensuring experimental consistency.

Reagents and Cell Culture Supplements

Sartorius provides a broad selection of reagents and supplements for maintaining cells, performing experiments, and analyzing results:

• animal component-free cell dissociation solution as a defined alternative to porcine trypsin
• recombinant human albumin
• human serum albumin solution optimized for human ES and iPS cell culture
• human platelet lysate – a cost-effective and superior alternative to FBS
• etc.