Classical media are the first generation of cell culture media and have been used in cell culture research for decades. They are a mixture of inorganic salts, amino acids, sugars, vitamins, and other components needed for optimal growth. They are typically used with serum and are multi-purpose, meaning they can be used to grow different cell lines.
Fetal bovine serum (FBS) is derived from the blood of fetal calves. It is depleted of cells, fibrin, and clotting factors while remaining serum contains many hormones and macromolecular factors essential for cell growth.
Fetal bovine serum (FBS) is a growth supplement for the culture of mammalian cells. FBS contains more than a thousand components, including proteins, electrolytes, lipids, carbohydrates, hormones, enzymes, and other undefined constituents, which are necessary for supporting cellular growth.