The Starting Kit of the 4Cell® XtraCHO Media System includes all the necessary medium components from adapting the cells to fed-batch production at a cultivation volume of 3L. For larger volumes all individual system components are available in different formats, in liquid (bottle and bag) and powder.
It allows the cultivation or media adaptation of cells. Lacking certain metabolites, the cultivation medium exerts a strong selection pressure on cells and shortens the time consuming adaptation to fed-batch culture.
It is a non-selective production medium for batch or fed-batch culture. It can be used for effective cultivation of recombinant CHO-DG44 cells and other CHO cell lines to produce high yield of the active protein.
Both feed additives are especially matched to the production medium. Combining those increases fed-batch productivity 3- to 5-fold compared to batch culture conditions.