PRC Prepacked Chromatographic Columns with MEP, HEA, and PPA HyperCel™ Mixed-Mode Sorbents

Quick overview of the selectivity of MEP, HEA, and PPA HyperCel mixed-mode sorbents

• Convenient – prepacked columns ready for use in 1 mL and 5 mL sizes
• Easy to use – direct connection to commonly used laboratory chromatographic systems
• Efficient – high packing efficiency (> 2500 plates/meter)
• Consistent – screening of mixed-mode chromatographic sorbents under reliable and reproducible conditions, ensuring batch-to-batch performance

HyperCel sorbents exploit unique selectivities of robust synthetic ligands to capture proteins or separate them from contaminants. They provide a unique separation mechanism different from conventional methods. All ligands operate predominantly by hydrophobic interaction. This “HIC-like” interaction typically occurs without adding lyotropic salt.

Applications

Rapid screening and condition optimization in the 1 mL PRC column enables rapid selection of the appropriate chemistry. Once the chemistry is selected on a 1 mL PRC column, the conditions of use can be optimized in a 5 mL PRC column in doubling the height.

Two or more columns can be connected in series to increase the column height and more closely model real conditions in pilot scale or for scale down applications.

Mixed-mode Chromatography Sorbents for Antibody Capture and “No Salt” Hydrophobic Interaction:

• MEP HyperCel sorbent: Antibody capture; alternative to conventional hydrophobic interaction
• MEP, HEA, PPA HyperCel sorbents: No salt/low salt alternative to hydrophobic interaction