Sartobind STIC® PA cassettes | 4 mm membrane

Sartobind STIC® PA removes contaminants with high salt concentrations. Salt-resistant interaction chromatography (STIC) with PA ligand is based on the principles of anion exchange (AEX). AEX is an established method for removing process-derived contaminants, such as host cell proteins (HCPs), DNA, endotoxins, and endogenous viruses. It is performed during the downstream process of monoclonal bodies (mAbs) inflow mode. Membranes are easy to manage and can be disposed of after a single-use, just like a filter, to save on validation costs.

Application:

Current downstream mAb processes consist of at least one initial binding and one step of AEX chromatography. The limitation of AEX steps is often that the Q membrane requires low-salt conditions. In cationic processes (CEX) as the second step of chromatography, the CEX set should be diluted to a conductivity of 4-7 mS/cm to establish the binding conditions for the next AEX step. The Sartobind STIC PA membrane consists of crosslinked, regenerated macroporous cellulose. The primary amine ligand is bound to the matrix at approximately six times the ligand density of that Q membrane. Free amine and high ligand density are crucial for the development of its high salt tolerance.

The Sartobind STIC® absorbent membrane accelerates the production process at high flow rates and opens up new possibilities for the purification of therapeutic proteins and vaccines.