The Incucyte® CX3 live-cell analysis system with confocal imaging enables crystal-clear visualization of organoids and spheroids. Thanks to its exceptional flexibility, the CX3 supports the simultaneous execution of 2D and 3D assays. Using both fluorescence-based and label-free methods for continuous monitoring of morphology, growth, and phenotypic changes, the system enables the collection of physiologically relevant data without disrupting cultures—providing clear insights that accelerate decision-making in drug development, disease modeling, and personalized therapies.