Incucyte® devices for monitoring and analyzing live cells are designed for efficient recording of cellular changes in the incubator. Capture high-resolution fluorescence and brightfield images, recording data in real-time over hours, days, or weeks. From proliferation assays to the immunological killing of tumor spheroids, this flexible system allows users to observe and quantify complex biological changes in real-time. The integrated software simplifies data analysis to accelerate response time, including the creation of publication-quality graphs and charts.
monitoring cell health and proliferation
investigating cell functions
tracking cell movement and morphology
monitoring the progress of cell culture
Comparison of device specifications
| Application |
Incucyte® SX5 |
Incucyte® S3 |
Incucyte® SX1 |
| Confluence | ✓ | ✓ | ✓ |
| Label-free Cell Couting* | ✓ | ✓ | ✓ |
| Cell Counting with Flourescent label | ✓ | ✓ | ✓ |
| Apoptosis^ | ✓ | ✓ | ✓ |
| Cytotoxicity | ✓ | ✓ | ✓ |
| Single Spheroid* | ✓ | ✓ | ✓ |
| Multi-Spheroid | ✓ | ✓ | ✓ |
| Cell Cycle^ | ✓ | ✓ | ✓ |
| ATP Metabolism* | ✓ | ||
| Mitochondrial Membrane Potential* | ✓ | ||
| See more | See more | See more |
*Requires software module.
*Additional software available for enhanced analysis.
| Application | SX5 | S3 | SX1 |
| Immune Cell Killing^ | ✓ | ✓ | ✓ |
| Antibody Internalization^ | ✓ | ✓ | ✓ |
| Phagcytosis | ✓ | ✓ | ✓ |
| NETosis | ✓ | ✓ | ✓ |
| Neuronal Activity* | ✓ | ||
| Angiogenesis* | ✓ | ✓ | ✓ |
| Live-Cell Immunocytochemistry^ | ✓ | ✓ | ✓ |
*Requires software module.
*Additional software available for enhanced analysis.
| Application | SX5 | S3 | SX1 |
| Chemotaxis* | ✓ | ✓ | ✓ |
| Scratch Wound* | ✓ | ✓ | ✓ |
| Immune Cell Clustering & Proliferation^ | ✓ | ✓ | ✓ |
| Neurite Outgrowth | ✓ | ✓ | ✓ |
*Requires software module.
*Additional software available for enhanced analysis.
| Application | SX5 | S3 | SX1 |
| Cell Culture QC | ✓ | ✓ | ✓ |
| Dilution Cloning | ✓ | ✓ | ✓ |
| Whole Well | ✓ | ✓ | ✓ |
| Reporter Genes^ | ✓ | ✓ | ✓ |
| Stem Cell Monitoring & Reprogramming | ✓ | ✓ | ✓ |
| Transfection Efficiency^ | ✓ | ✓ | ✓ |
*Requires software module.
*Additional software available for enhanced analysis.
| Specification | SX5 | S3 | SX1 |
| Interchangeable Trays | 3 | 3 | 1 |
| Microplate Capacity | 6 | 6 | 2 |
| Microplate Compatibility | 6, 12, 24, 48, 96 & 384 well (1) | ||
| Flask Compatibility | T-25, T-50, T-75, T-100, T-175, T-225 (2) | ||
| Other Labware Compatibility | 35mm, 60mm, 100mm, 150mm dishes, and chamber slides (2) | ||
(1) 3 microplate trays included for SX5 and S3. Purchase separately for SX1.
(2) Trays available for purchase separately. See our latest vessel compatibility list for full details.
| Specification | SX5 | S3 | SX1 |
| Total Fluorescence Channels | Up to 5 | 2 | 2 |
| -Green/Orange/NIR Optical Module | included | N/A | N/A |
| -Green/Red Optical Module | optional | included | included |
| -Metabolism Optical Module | optional | N/A | N/A |
| Label-Free, HD Phase Imaging | ✓ | ✓ | ✓ |
| Objectives | 4x, 10x, 20x included | 4x, 10x, or 20x purchase separately | |
| Objective Automation | Automated turret | Manual setting in software to switch |
| Specification | SX5 | S3 | SX1 |
| Remote Network Capability | ✓ | ✓ | ✓ |
| Unlimited, free user licenses | ✓ | ✓ | ✓ |
| Independent Vessel Scheduling | ✓ | ✓ | N/A |
| Multi-objective Scheduling | ✓ | ✓ | N/A |
| Specification | SX5 | S3 | SX1 |
| Incubation | All systems leverage the stable incubation of a tissue culture incubator | ||
| Incubator size | >200 L required | >200 L recommended | >200 L recommended |
| Incubation to 42°C | ✓ | ✓ | ✓ |
| Specification | SX5 | S3 | SX1 |
| Operating System | 64-bit Windows 10 | ||
| Controller Data Storage | 27.3 TB | 16.4 TB | 16.4 TB |
| Expandable RAID Storage | Add an additional 32.7 TB of storage with Incustore | ||
| Controller Memory | 64 GB | 48 GB | 48 GB |
*All software modules sold separately.
| Specification | SX5 | S3 | SX1 |
| Incucyte® Cell-by-Cell | ✓ | ✓ | ✓ |
| Incucyte® Spheroid | ✓ | ✓ | ✓ |
| Incucyte® Cell Migration | ✓ | ✓ | ✓ |
| Incucyte® Chemotaxis | ✓ | ✓ | ✓ |
| Incucyte® Neurotrack | ✓ | ✓ | ✓ |
| Incucyte® Neuronal Activity | ✓ | ||
| Incucyte® Angiogenesis | ✓ | ✓ | ✓ |
| Incucyte® ATP Analysis NEW | ✓ |
