Filtration is a key technique in the laboratory. From reagent sterilization and basic research to clinical and environmental testing, filtration is one of the fundamental steps in preparing samples for further analysis. When choosing the right filtration system, it is important to take into account certain factors that can affect the final outcome of filtration - from material compatibility to pore size. Below is a quick guide to laboratory filtration and the basic factors you should consider when choosing and purchasing a laboratory filtration system.
The pore size determines the size range of particles that can pass through the filter or retain them. The exact pore size required for a particular application will depend on the specific sample, purity and yield required. In some cases, multiple filtration steps or the use of filters with different pore sizes may be necessary to obtain the desired result.
In order to achieve the best yield and purity, the filter material should be chemically and physically compatible with the content and composition of the sample being filtered. For example, some filter housings are more chemically resistant than others. In addition, some filters may be considered "sticky" to certain proteins; this means that proteins will adsorb to the membrane, reducing the final yield. Finally, filter material that is not compatible with process conditions, such as operating temperature, pressure, and pH, can shrink, expand or deform, which can ultimately lead to leakage or a change in filter pore size.
The flow rate is the speed at which the liquid or gas passes through the filter. It depends on a variety of factors, including:
While a higher flow rate can speed things up, it can also retain less of the material you want, affecting the purity and yield of your final product. In order to get the best result, it is important to find the optimal balance between flow and retention.
Depending on the application, you may need different types of filtration products. For example, syringe filters are small, disposable filters commonly used for laboratory filtration for sample preparation, sterilization and clarification of aqueous solutions, solvents, and other liquids. Clarification filters are designed to remove larger particles and impurities from liquids and can be used in a variety of work processes, including cell culture. In-line filters are another type of filtration product that removes contaminants and particles as the fluid flows through the system.
In our range, we offer a wide range of laboratory filtration and purification products for a number of applications, including basic research, drug discovery, analytics and diagnostic testing. Discover our depth filters, microporous membranes, syringe filters, clarification filters, line filters and other products.
The original text is from the official Sartorius page.